PRODUCT CODE: EM1701-71

PRDX2 Monoclonal Antibody (EM1701-71)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of Peroxiredoxin 2 on PC-3M (1) and MCF-7 (2) using anti- Peroxiredoxin 2 antibody at 1/5,000 dilution.
  • Western blot analysis of Peroxiredoxin 2 on PC-3M (1) and MCF-7 (2) using anti- Peroxiredoxin 2 antibody at 1/5,000 dilution.
  • ICC staining Peroxiredoxin 2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Peroxiredoxin 2 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of PC-3M cells with Peroxiredoxin 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of Peroxiredoxin 2 on PC-3M (1) and MCF-7 (2) using anti- Peroxiredoxin 2 antibody at 1/5,000 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PRDX2 Monoclonal Antibody (EM1701-71)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

PC-3M, MCF-7, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7F4

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:2,000-1:10,000

  • ICC

  • 1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PRDX2

SYNONYMS

Epididymis secretory sperm binding protein Li 2a antibody; HEL S 2a antibody; MGC4104 antibody; Natural killer cell enhancing factor B antibody; Natural killer cell-enhancing factor B antibody; Natural Killer Enhancing Factor B antibody; NKEF B antibody; NKEF-B antibody; NKEFB antibody; Peroxiredoxin-2 antibody; PRDX 2 antibody; PRDX2 antibody; PRDX2_HUMAN antibody; PrP antibody; PRX2 antibody; PRXII antibody; PTX1 antibody; TDPX1 antibody; Thiol Specific Antioxidant 1 antibody; Thiol specific antioxidant protein antibody; Thiol-specific antioxidant protein antibody; Thioredoxin Dependent Peroxide Reductase 1 antibody; Thioredoxin peroxidase 1 antibody; Thioredoxin-dependent peroxide reductase 1 antibody; Torin antibody; TPX1 antibody; TSA antibody

SEQUENCE SIMILARITIES

Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.

POST-TRANSLATIONAL MODIFICATION

The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX I gene encodes a protein that is expressed in several tissues, including liver, kidney, testis, lung and nervous system. PRX II is expressed in testis, while PRX III shows expression in lung. PRX I, II and III are overexpressed in breast cancer and may be involved in its development or progression. Upregulated protein levels of PRX I and II in Alzheimer's disease (AD) and Down syndrome (DS) indicate the involvement of PRX I and II in their pathogenesis.