PPP2R1B Recombinant Rabbit Monoclonal Antibody [PSH02-92] (HA721911)
Catalog# HA721911
Rabbit Monoclonal to PPP2R1B
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PPP2R1B on different lysates with Rabbit anti-PPP2R1B antibody (HA721911) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Caco-2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse testis tissue lysate
Lane 7: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721911) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Caco-2 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse testis tissue lysate
Lane 7: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721911) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
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