PRODUCT CODE: ER1803-30

PPAR gamma Rabbit Polyclonal Antibody (ER1803-30)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PPAR gamma on PC-12 cell and human liver tissue lysate using anti-PPAR gamma antibody at 1/500 dilution.
  • Western blot analysis of PPAR gamma on PC-12 cell and human liver tissue lysate using anti-PPAR gamma antibody at 1/500 dilution.
  • ICC staining PPAR gamma in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PPAR gamma in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PPAR gamma in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PPAR gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PPAR gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-PPAR gamma antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with PPAR gamma antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of PPAR gamma on PC-12 cell and human liver tissue lysate using anti-PPAR gamma antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PPAR gamma Rabbit Polyclonal Antibody (ER1803-30)

Immunogen

Recombinant protein within human ppar gamma aa 250-450.

Host

Rabbit

Positive Control

PC-12, human liver tissue lysate, A431, A549, JAR, human lung cancer tissue, human colon tissue, mouse uterus tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

57/54/21 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PPAR gamma

SYNONYMS

CIMT1 antibody; GLM1 antibody; NR1C3 antibody; Nuclear receptor subfamily 1 group C member 3 antibody; OTTHUMP00000185032 antibody; OTTHUMP00000185036 antibody; Peroxisome proliferator activated nuclear receptor gamma variant 1 antibody; Peroxisome proliferator activated receptor gamma 1 antibody; Peroxisome Proliferator Activated Receptor gamma antibody; Peroxisome proliferator-activated receptor gamma antibody; PPAR gamma antibody; PPAR-gamma antibody; PPARG antibody; PPARG_HUMAN antibody; PPARG1 antibody; PPARG2 antibody; PPARgamma antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR1 subfamily.

TISSUE SPECIFICITY

Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.

POST-TRANSLATIONAL MODIFICATION

O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes.; Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity (By similarity).

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs). These heterodimers regulate transcription of genes involved in insulin action, adipocyte differentiation, lipid metabolism and inflammation. PPARγ is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPARγ activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPARγ may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPARγ2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPARγ2 gene maybe correlated with abdominal obesity in type 2 diabetes.