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PRODUCT CODE: ER1706-37

PP2C alpha Antibody (ER1706-37)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.
  • Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.
  • ICC staining PP2C alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PP2C alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PP2C alpha in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with PP2C alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PP2C alpha Antibody (ER1706-37)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse kidney tissue lysate, rat skin tissue lysate, A431, A549, SH-SY5Y, human kidney tissue, human colon cancer tissue, mouse intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

42/35 kDa, additional band 55 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P35813

PROTEIN NAME

PP2C alpha

SYNONYMS

Protein phosphatase 2C isoform alpha antibody; EC 3.1.3.16 antibody; FLJ42306 antibody IA antibody; MGC9201 antibody; Mpp alpha antibody; PP2C alpha antibody; PP2C-alpha antibody; PP2CA antibody; PP2Calpha antibody; PPM 1A antibody; PPM1A antibody; PPM1A_HUMAN antibody; PPPM1A antibody; Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody; Protein phosphatase 1A antibody; Protein phosphatase 1A magnesium dependent alpha antibody; Protein phosphatase 2C alpha antibody; Protein phosphatase 2C alpha isoform antibody; Protein phosphatase 2C isoform alpha antibody; Protein phosphatase IA antibody; Protein phosphatase, Mg2+/Mn2+ dependent, 1A antibody

SEQUENCE SIMILARITIES

Belongs to the PP2C family.

POST-TRANSLATIONAL MODIFICATION

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.

SUBCELLULAR LOCATION

Cytoplasm, Membrane, Nucleus.

FUNCTION

The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain.