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PRODUCT CODE: ER1706-37

PP2C alpha Antibody (ER1706-37)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.
  • Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.
  • ICC staining PP2C alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PP2C alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PP2C alpha in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A431 cells with PP2C alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PP2C alpha Antibody (ER1706-37)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse kidney tissue lysate, rat skin tissue lysate, A431, A549, SH-SY5Y, human kidney tissue, human colon cancer tissue, mouse intestine tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

42/35 kDa, additional band 55 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P35813

PROTEIN NAME

Protein phosphatase 1A

GENE NAME

PPM1A

SYNONYMS

PP2C-alpha, PPM1A, PPPM1A

SEQUENCE SIMILARITIES

Belongs to the PP2C family.

POST-TRANSLATIONAL MODIFICATION

N-myristoylation is essential for the recognition of its substrates for dephosphorylation.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm, cytosol. Membrane; Lipid-anchor. Note=Weakly associates at the membrane and N-myristoylation mediates the membrane localization.

FUNCTION

Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.