PRODUCT CODE: em1701-90

PLGF Monoclonal Antibody (EM1701-90)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.
  • Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.
  • ICC staining PLGF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PLGF (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PLGF Monoclonal Antibody (EM1701-90)

Immunogen

Recombinant protein within human plgf 1-150 aa.

Host

Mouse

Positive Control

JAR, HepG2, LOVO, rat kidney tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3G2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Placenta growth factor

GENE NAME

PGF

SYNONYMS

PlGF, PGF, PGFL, PLGF

SEQUENCE SIMILARITIES

Belongs to the PDGF/VEGF growth factor family.

TISSUE SPECIFICITY

While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.

FUNCTION

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.