PRODUCT CODE: EM1701-90

PLGF Monoclonal Antibody (EM1701-90)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.
  • Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.
  • ICC staining PLGF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PLGF (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PLGF Monoclonal Antibody (EM1701-90)

Immunogen

Recombinant protein within human plgf 1-150 aa.

Host

Mouse

Positive Control

JAR, HepG2, LOVO, rat kidney tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3G2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PLGF

SYNONYMS

D12S1900 antibody; Pgf antibody; PGFL antibody; PIGF antibody; Placenta growth factor antibody; Placental growth factor antibody; Placental growth factor, vascular endothelial growth factor related protein antibody; PlGF 2 antibody; PlGF antibody; PLGF_HUMAN antibody; PlGF2 antibody; SHGC 10760 antibody

SEQUENCE SIMILARITIES

Belongs to the PDGF/VEGF growth factor family.

TISSUE SPECIFICITY

While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factor (FGF), platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). Like VEGF, several PlGF variants have been shown to arise from alternative mRNA splicings. Evidence has suggested VEGF to be an obligatory component in PlGF signaling. While VEGF homodimers and VEGF/PlGF heterodimers function as potent mediators of mitogenic and chemotactic responses in endothelial cells, PlGF homodimers are effectual only at extremely high concentrations. Indeed, many of the physiological effects attributed to VEGF may actually be a result of VEGF/PlGF. VEGF and PlGF share a common receptor, Flt-1, and may also activate Flk-1/KDR.