PRODUCT CODE: em1701-88

PLGF Monoclonal Antibody (EM1701-88)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.
  • Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.
  • ICC staining PLGF (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PLGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PLGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PLGF antibody. Counter stained with hematoxylin.
Western blot analysis of PLGF on JAR using anti-PLGF antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PLGF Monoclonal Antibody (EM1701-88)

Immunogen

Recombinant protein within human plgf 1-150 aa.

Host

Mouse

Positive Control

JAR, LOVO, rat kidney tissue, human placenta tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3G3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50 IHC-P

TARGET

UNIPROT #

PROTEIN NAME

Placenta growth factor

GENE NAME

PGF

SYNONYMS

PlGF, PGF, PGFL

SEQUENCE SIMILARITIES

Belongs to the PDGF/VEGF growth factor family.

TISSUE SPECIFICITY

While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Secreted. Note=The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.

FUNCTION

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.