PRODUCT CODE: ER1706-27

PINK1 Antibody (ER1706-27)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of PINK1 on different lysates using anti-PINK1 antibody at 1/200 dilution.<br />
Positive control:<br />
Lane 1: A431 cell lysates<br />
Lane 2: Mouse testis tissue lysates<br />
Lane 3: Jurkat cell lysates
  • Western blot analysis of PINK1 on different lysates using anti-PINK1 antibody at 1/200 dilution.<br />
Positive control:<br />
Lane 1: A431 cell lysates<br />
Lane 2: Mouse testis tissue lysates<br />
Lane 3: Jurkat cell lysates
  • ICC staining PINK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PINK1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PINK1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PINK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PINK1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-PINK1 antibody. Counter stained with hematoxylin.
Western blot analysis of PINK1 on different lysates using anti-PINK1 antibody at 1/200 dilution.
Positive control:
Lane 1: A431 cell lysates
Lane 2: Mouse testis tissue lysates
Lane 3: Jurkat cell lysates

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PINK1 Antibody (ER1706-27)

Immunogen

Recombinant protein within human pink1 aa 146-300.

Host

Rabbit

Positive Control

A431, mouse testis tissue lysate, Jurkat, Hela, PC-3M, SK-Br-3, human breast tissue, human placenta tissue, human stomach cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

63 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Serine/threonine-protein kinase PINK1, mitochondrial

GENE NAME

PINK1

SYNONYMS

PINK1

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

TISSUE SPECIFICITY

Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.; Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. Processed into a 52 kDa mature form by PARL or AFG3L2 following the cleavage by mitochondrial-processing peptidase (MPP) during mitochondrial import. In depolarized mitochondria, cleaved by OMA1 that acts as a backup protease, promoting its subsequent degradation by the proteasome.

SUBCELLULAR LOCATION

Mitochondrion outer membrane; Single-pass membrane protein. Note=Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol. When mitochondria lose mitochondrial membrane potential following damage, PINK1 import is arrested, which induces its accumulation in the outer mitochondrial membrane, where it acquires kinase activity.

FUNCTION

Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN. Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form. Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity).