PRODUCT CODE: ER2001-09

PHR2 Rabbit Polyclonal Antibody (ER2001-09)

Applications

  • WB

  • IHC

REACTIVITY

  • Rice

Western blot analysis of PHR2 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PHR2 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded rice tissue using anti-PHR2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-09, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of PHR2 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC

REACTIVITY

  • Rice

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PHR2 Rabbit Polyclonal Antibody (ER2001-09)

Immunogen

Recombinant protein within rice phr2 aa 1-300.

Host

Rabbit

Positive Control

Rice tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size: 46 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PHR2

SYNONYMS

Protein PHOSPHATE STARVATION RESPONSE 2 antibody; OsPHR2 antibody ; PHR2 antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Transcription factor involved in phosphate starvation signaling . Binds to P1BS, an imperfect palindromic sequence 5'-GNATATNC-3', to promote the expression of inorganic phosphate (Pi) starvation-responsive genes . Functionally redundant with PHR1 and PHR3 in regulating Pi starvation response and Pi homeostasis . Involved in both systematic and local Pi-signaling pathways. Regulates several Pi transporters . Regulates the expression of PT2 . Directly up-regulates SPX1 and SPX2 expression, but PHR2 binding to DNA is repressed redundantly by SPX1 and SPX2 in a PI-dependent manner . The DNA-binding activity is also repressed by SPX4. Involved in root growth under Pi deprivation.