Phospho-Glycogen synthase 1 (S641) Recombinant Rabbit Monoclonal Antibody [SR46-06] (ET1602-13)
Catalog# ET1602-13
Rabbit Monoclonal to Phospho-Glycogen synthase 1 (S641)
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Mouse
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Rat
Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: A431 cell lysate
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-Glycogen synthase 1 (S641) Recombinant Rabbit Monoclonal Antibody [SR46-06] (ET1602-13)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding ser641 of human glycogen synthase 1.
Host
Rabbit
Modification
Phospho
Modification Site
S641
Positive Control
SK-Br-3 cell lysate, A431 cell lysate, HepG2 cell lysate, Mouse liver tissue lysate, A549, NIH/3T3, mouse liver tissue, mouse skeletal muscle tissue, mouse smooth muscle tissue, rat skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SR46-06
RRID
APPLICATION DILUTION
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WB
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1:500
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IF-Cell
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1:50
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 84 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-Glycogen synthase 1 (S641)
SYNONYMS
Glycogen [starch] synthase antibody;Glycogen synthase 1 (muscle) antibody;Glycogen synthase 1 antibody;GSY antibody;GYS antibody;Gys1 antibody;GYS1_HUMAN antibody;muscle antibody
SEQUENCE SIMILARITIES
Belongs to the glycosyltransferase 3 family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B (By similarity). Phosphorylated at Ser-641 by DYRK2, leading to inactivation (By similarity). Phosphorylated at Ser-641 by PASK, leading to inactivation; phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
SUBCELLULAR LOCATION
Cytoplasm
FUNCTION
Glycogen [starch] synthase belongs to the mammalian/fungal glycogen synthase family of proteins. Two forms of this protein exist, a liver form and a muscle form, both of which have the same function in the glycogen biosynthesis pathway. Glycogen synthase transfers the glycosyl residue from UDP-Glucose to the nonreducing end of ?-1,4-glucan. The liver glycogen synthase protein is truncated by 34 amino acids compared to the muscle form. However, these enzymes differ significantly in their amino- and carboxyl-terminal regions. Muscle glycogen synthase serves to fuel muscular activity only and is regulated by muscle contraction and by catecholamines. Liver glycogen synthase mediates blood glucose homeostasis in response to nutritional cues. Defects in the gene encoding liver glycogen synthase results in glycogen storage disease type 0 (GSD0), a rare form of fasting ketotic hypoglycemia.
CITATIONS
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Shu, Y., Zou, C., Cai, Y., He, Q., Wu, X., Zhu, H., Qv, M., Chao, Y., Xu, C., Tang, L., & Wu, X.
Vitamin C deficiency induces hypoglycemia and cognitive disorder through S-nitrosylation-mediated activation of glycogen synthase kinase 3β
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WB
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Mouse
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Citation
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