PRODUCT CODE: ER1511-14

PHF10 Antibody (ER1511-14)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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+
Western blot analysis of PHF10 on Hela cell lysate using anti-PHF10 antibody at 1/1,000 dilution.
  • Western blot analysis of PHF10 on Hela cell lysate using anti-PHF10 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-PHF10 antibody. Counter stained with hematoxylin.
Western blot analysis of PHF10 on Hela cell lysate using anti-PHF10 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PHF10 Antibody (ER1511-14)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, rat testis tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PHF10

SYNONYMS

BAF45a antibody; BRG1-associated factor 45a antibody; FLJ10975 antibody; MGC111009 antibody; PHD finger protein 10 antibody; PHD finger protein 10, isoform a antibody; PHD zinc finger protein XAP135 antibody; Phf10 antibody; PHF10_HUMAN antibody; XAP135 antibody

SEQUENCE SIMILARITIES

Belongs to the SAYP family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.