PRODUCT CODE: em1701-86

PGP9.5 Monoclonal Antibody (EM1701-86)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PGP9.5 on A172 (1) and SHG-44 (2) using anti-PGP9.5 antibody at 1/500 dilution.
  • Western blot analysis of PGP9.5 on A172 (1) and SHG-44 (2) using anti-PGP9.5 antibody at 1/500 dilution.
  • ICC staining PGP9.5 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PGP9.5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with PGP9.5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of PGP9.5 on A172 (1) and SHG-44 (2) using anti-PGP9.5 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PGP9.5 Monoclonal Antibody (EM1701-86)

Immunogen

Synthetic peptide within c-terminal human pgp9.5.

Host

Mouse

Positive Control

A172, SHG-44, A549, SH-SY5Y, rat cerebellum tissue, human kidney tissue, human pancreas tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B1-5-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Ubiquitin carboxyl-terminal hydrolase isozyme L1

GENE NAME

UCHL1

SYNONYMS

UCH-L1, PGP9.5, UCHL1

SEQUENCE SIMILARITIES

Belongs to the peptidase C12 family.

TISSUE SPECIFICITY

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated.

SUBCELLULAR LOCATION

Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain.

FUNCTION

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity.