PRODUCT CODE: ER40404

PDI Antibody (ER40404)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Hela&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 2: MCF-7<br />
Lane 3: PANC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 4: HepG2<br />
Lane 5: HUVEC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 6: NIH/3T3<br />
Lane 7: L929&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 8: F9<br />
Lane 9: Jurkat&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 10: A431<br />
Lane 11: A549&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 12: Mouse liver<br />
Lane 13: Mouse kidney&nbsp;&nbsp;&nbsp;Lane 14: Mouse brain<br />
Lane 15: Human kidney&nbsp;&nbsp;&nbsp;Lane 16: Human brain<br />
Lane 17: Human liver
  • Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.<br />
Positive control: <br />
Lane 1: Hela&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 2: MCF-7<br />
Lane 3: PANC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 4: HepG2<br />
Lane 5: HUVEC&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 6: NIH/3T3<br />
Lane 7: L929&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 8: F9<br />
Lane 9: Jurkat&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 10: A431<br />
Lane 11: A549&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Lane 12: Mouse liver<br />
Lane 13: Mouse kidney&nbsp;&nbsp;&nbsp;Lane 14: Mouse brain<br />
Lane 15: Human kidney&nbsp;&nbsp;&nbsp;Lane 16: Human brain<br />
Lane 17: Human liver
  • ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PDI in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela     Lane 2: MCF-7
Lane 3: PANC     Lane 4: HepG2
Lane 5: HUVEC     Lane 6: NIH/3T3
Lane 7: L929     Lane 8: F9
Lane 9: Jurkat     Lane 10: A431
Lane 11: A549     Lane 12: Mouse liver
Lane 13: Mouse kidney   Lane 14: Mouse brain
Lane 15: Human kidney   Lane 16: Human brain
Lane 17: Human liver

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PDI Antibody (ER40404)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, MCF-7, PANC,HepG2, HUVEC, NIH/3T3, L929, F9, Jurkat, A431, A549, mouse liver tissue, mouse kidney tissue, mouse brain tissue,human lung carcinoma tissue,human kidney,Human brain,Human liver,human colon carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Protein disulfide-isomerase A2

GENE NAME

PDIA2

SYNONYMS

PDIp

SEQUENCE SIMILARITIES

Belongs to the protein disulfide isomerase family.

TISSUE SPECIFICITY

Highly expressed in pancreas (at protein level).

POST-TRANSLATIONAL MODIFICATION

The disulfide-linked homodimer exhibits an enhanced chaperone activity.; Glycosylated.

SUBCELLULAR LOCATION

Endoplasmic reticulum lumen.

FUNCTION

Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins.