PRODUCT CODE: EM1707-60

PD1 Monoclonal Antibody (EM1707-60)

  • IVD–IHC

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of PD1 on PD1 transfected 293FT cell lysate using anti-PD1 antibody at 1/1,000 dilution.
  • Western blot analysis of PD1 on PD1 transfected 293FT cell lysate using anti-PD1 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with PD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of PD1 on PD1 transfected 293FT cell lysate using anti-PD1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PD1 Monoclonal Antibody (EM1707-60)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Human tonsil tissue, Mouse spleen tissue, Jurkat, recombinant PD1 protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1F2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PD1

SYNONYMS

CD279 antibody; CD279 antigen antibody; hPD 1 antibody; hPD l antibody; hPD-1 antibody; hSLE1 antibody; PD 1 antibody; PD-1 antibody; PD1 antibody; PDCD 1 antibody; PDCD1 antibody; PDCD1_HUMAN antibody; Programmed cell death 1 antibody; Programmed cell death 1 protein antibody; Programmed cell death protein 1 antibody; Protein PD 1 antibody; Protein PD-1 antibody; SLEB2 antibody; Systemic lupus erythematosus susceptibility 2 antibody

DEVELOPMENTAL STAGE

Induced at programmed cell death.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated at Lys-233 by the SCF(FBXO38) complex, leading to its proteasomal degradation. Ubiquitinated via 'Lys-48'-linked polyubiquitin chains.; Tyrosine phosphorylated at Tyr-223 (within ITIM motif) and Tyr-248 (ITSM motif) upon ligand binding. Phosphorylation at Tyr-248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.; N-glycosylation at Asn-58 consists of two N-acetylglucosamine units and one fucose. N-glycosylation does not affect binding to nivolumab drug.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Pdcd-1 (Programmed Cell Death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. Pdcd-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) within the cytoplasmic domain, which is conserved between the mouse and human homologs. Expression of Pdcd-1 is detected in mouse thymus, and it is induced in stimulated B and T cell lines, where it may play a role in the negative regulation of various immune responses. Receptors such as Pdcd-1 function by recruiting tyrosine phosphatases, including SHP-1 and SHIP, which are responsible for altering various B cell responses. Additionally, in activated lymphocytes, Pdcd-1 mediates the activation of the classical type of programmed cell death.