PRODUCT CODE: R1608-6

PCBP1 Antibody (R1608-6)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of PCBP1 on different cell lysate using anti-PCBP1 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: F9 <br />
  Lane 2: NIH-3T3 Lane 3: K562
  • Western blot analysis of PCBP1 on different cell lysate using anti-PCBP1 antibody at 1/1,000 dilution.<br />
  Positive control:<br />
  Lane 1: F9 <br />
  Lane 2: NIH-3T3 Lane 3: K562
  • ICC staining PCBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PCBP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PCBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PCBP1 antibody. Counter stained with hematoxylin.
Western blot analysis of PCBP1 on different cell lysate using anti-PCBP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: F9
Lane 2: NIH-3T3 Lane 3: K562

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PCBP1 Antibody (R1608-6)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

F9, K562, NIH-3T3, A549, HepG2, MCF-7, human spleen tissue, mouse testis tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PCBP1

SYNONYMS

Alpha-CP1 antibody; Heterogeneous nuclear ribonucleoprotein E1 antibody; heterogenous nuclear ribonucleoprotein E1 antibody; heterogenous nuclear ribonucleoprotein X antibody; hnRNP E1 antibody; hnRNP-E1 antibody; hnRNP-X antibody; HNRPE1 antibody; HNRPX antibody; nucleic acid binding protein sub 2.3 antibody; Nucleic acid- binding protein SUB2.3 antibody; Nucleic acid-binding protein SUB2.3 antibody; PCBP1 antibody; PCBP1_HUMAN antibody; poly(rC) binding protein 1 antibody; Poly(rC)-binding protein 1 antibody; rCbinding protein 1 antibody

TISSUE SPECIFICITY

Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated; lowers poly(rC)-binding activity.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. In case of infection by poliovirus, plays a role in initiation of viral RNA replication in concert with the viral protein 3CD. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.