PRODUCT CODE: ER1802-51

Pax8 Antibody (ER1802-51)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Pax8 on human thyroid gland tissue and SKOV-3 cell lysates using anti-Pax8 antibody at 1/500 dilution.
  • Western blot analysis of Pax8 on human thyroid gland tissue and SKOV-3 cell lysates using anti-Pax8 antibody at 1/500 dilution.
  • ICC staining Pax8 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Pax8 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SiHa cells with Pax8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Pax8 on human thyroid gland tissue and SKOV-3 cell lysates using anti-Pax8 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Pax8 Antibody (ER1802-51)

Immunogen

Synthetic peptide within human pax8 aa 400-450.

Host

Rabbit

Positive Control

SKOV-3, human thyroid gland tissue, SiHa.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Paired box protein Pax-8

GENE NAME

PAX8

SYNONYMS

PAX8

TISSUE SPECIFICITY

Expressed in the excretory system, thyroid gland and Wilms tumors.

DEVELOPMENTAL STAGE

In developing excretory system, during thyroid differentiation and in adult thyroid.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.