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PRODUCT CODE: ER1902-29

PAS2 Antibody (ER1902-29)

Applications

  • IF

  • IHC-P

REACTIVITY

  • Arabidopsis thaliana

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Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-29 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
  • Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-29 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-PAS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-29 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.

Applications

  • IF

  • IHC-P

REACTIVITY

  • Arabidopsis thaliana

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PAS2 Antibody (ER1902-29)

Immunogen

Synthetic peptide within a. thaliana pas2 aa 180-221.

Host

Rabbit

Positive Control

A. thaliana tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG

APPLICATION DILUTION

  • IF:1:50-1:100

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

Q8VZB2

PROTEIN NAME

PAS2

SYNONYMS

Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2Curated; 3-hydroxyacyl-CoA dehydratase PASTICCINO 2; AtPAS2; HACD; HCD; Protein PEPINO; Protein tyrosine phosphatase-like protein; PAS2; PEP; At5g10480; F12B17.170

SEQUENCE SIMILARITIES

Belongs to the very long-chain fatty acids dehydratase HACD family.

TISSUE SPECIFICITY

High expression in young seedlings, roots, root tips, flowers and young siliques. Lower levels in leaves and stems.

SUBCELLULAR LOCATION

Nucleus, endoplasmic reticulum membrane, cytoplasm.

FUNCTION

Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May be an anti-phosphatase that prevents CDKA-1 dephosphorylation and activation. Involved in the hormonal control of cell division and differentiation. Required for proliferation control of meristematic and non-meristematic cells. Negative regulator of the cell cycle.