Applications
-
IF
-
IHC-P
REACTIVITY
-
Arabidopsis thaliana
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
PAS2 Antibody (ER1902-29)
Immunogen
Synthetic peptide within a. thaliana pas2 aa 180-221.
Host
Rabbit
Positive Control
A. thaliana tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
25 kDa
Isotype
IgG
APPLICATION DILUTION
-
IF:1:50-1:100
-
IHC-P:1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
PAS2
SYNONYMS
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase PASTICCINO 2Curated; 3-hydroxyacyl-CoA dehydratase PASTICCINO 2; AtPAS2; HACD; HCD; Protein PEPINO; Protein tyrosine phosphatase-like protein; PAS2; PEP; At5g10480; F12B17.170
SEQUENCE SIMILARITIES
Belongs to the very long-chain fatty acids dehydratase HACD family.
TISSUE SPECIFICITY
High expression in young seedlings, roots, root tips, flowers and young siliques. Lower levels in leaves and stems.
SUBCELLULAR LOCATION
Nucleus, endoplasmic reticulum membrane, cytoplasm.
FUNCTION
Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May be an anti-phosphatase that prevents CDKA-1 dephosphorylation and activation. Involved in the hormonal control of cell division and differentiation. Required for proliferation control of meristematic and non-meristematic cells. Negative regulator of the cell cycle.