PRODUCT CODE: EM1701-16

PARP1 Mouse Monoclonal Antibody [A0-D11] (EM1701-16)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PARP1 on different lysates using anti-PARP1 antibody at 1/100 dilution.<br />
Positive control:<br />
Lane 1: Daudi<br />
Lane 2: Rat spleen tissue
  • Western blot analysis of PARP1 on different lysates using anti-PARP1 antibody at 1/100 dilution.<br />
Positive control:<br />
Lane 1: Daudi<br />
Lane 2: Rat spleen tissue
  • ICC staining PARP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Daudi cells with PARP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of PARP1 on different lysates using anti-PARP1 antibody at 1/100 dilution.
Positive control:
Lane 1: Daudi
Lane 2: Rat spleen tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PARP1 Mouse Monoclonal Antibody [A0-D11] (EM1701-16)

Immunogen

Peptide

Host

Mouse

Positive Control

Daudi, rat spleen tissue lysate, rat brain tissue, human tonsil tissue, human pancreas tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A0-D11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

113 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:100

  • IHC-P

  • 1:100-1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PARP1

SYNONYMS

ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody; ADP ribosyltransferase antibody; ADP ribosyltransferase diphtheria toxin like 1 antibody; ADP ribosyltransferase NAD(+) antibody; ADPRT 1 antibody; ADPRT antibody; ADPRT1 antibody; ARTD1 antibody; msPARP antibody; NAD(+) ADP ribosyltransferase 1 antibody; NAD(+) ADP-ribosyltransferase 1 antibody; pADPRT 1 antibody; pADPRT1 antibody; PARP 1 antibody; PARP antibody; PARP-1 antibody; PARP1 antibody; PARP1_HUMAN antibody; Poly (ADP ribose) polymerase 1 antibody; poly (ADP ribose) polymerase family, member 1 antibody; Poly [ADP-ribose] polymerase 1 antibody; Poly(ADP ribose) polymerase antibody; poly(ADP ribose) synthetase antibody; poly(ADP ribosyl)transferase antibody; Poly[ADP ribose] synthetase 1 antibody; Poly[ADP-ribose] synthase 1 antibody; PPOL antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PRKDC and TXK.; Poly-ADP-ribosylated on glutamate and aspartate residues by autocatalysis. Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1.; S-nitrosylated, leading to inhibit transcription regulation activity.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity.