PRODUCT CODE: ER31205

p70 S6 Kinase beta 1 Rabbit Polyclonal Antibody (ER31205)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of p70 S6 Kinase beta on different cell lysates using anti-p70 S6 Kinase beta antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: PC12             <br />
Lane 2: NIH/3T3 <br />
Lane 3: Jurkat           <br />
Lane 4: K562 <br />
Lane 5: Hela
  • Western blot analysis of p70 S6 Kinase beta on different cell lysates using anti-p70 S6 Kinase beta antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: PC12             <br />
Lane 2: NIH/3T3 <br />
Lane 3: Jurkat           <br />
Lane 4: K562 <br />
Lane 5: Hela
  • ICC staining p70 S6 Kinase beta in  Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining p70 S6 Kinase beta in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining p70 S6 Kinase beta in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti- p70 S6 Kinase beta antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with p70 S6 Kinase beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of p70 S6 Kinase beta on different cell lysates using anti-p70 S6 Kinase beta antibody at 1/500 dilution.
Positive control:
Lane 1: PC12
Lane 2: NIH/3T3
Lane 3: Jurkat
Lane 4: K562
Lane 5: Hela

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

p70 S6 Kinase beta 1 Rabbit Polyclonal Antibody (ER31205)

Immunogen

Synthetic peptide within human p70 s6 kinase beta 1 c terminal.

Host

Rabbit

Positive Control

PC12, Hela, NIH/3T3, Jurkat, HepG2, K562, Lovo, mouse pancreas tissue, rat pancreas tissue, human breast cancer tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

p70 S6 Kinase beta 1

SYNONYMS

70 kDa ribosomal protein S6 kinase 1 antibody; KS6B1_HUMAN antibody; p70 alpha antibody; P70 beta 1 antibody; p70 ribosomal S6 kinase alpha antibody; p70 ribosomal S6 kinase beta 1 antibody; p70 S6 kinase alpha antibody; P70 S6 Kinase antibody; p70 S6 kinase, alpha 1 antibody; p70 S6 kinase, alpha 2 antibody; p70 S6K antibody; p70 S6K-alpha antibody; p70 S6KA antibody; p70(S6K) alpha antibody; p70(S6K)-alpha antibody; p70-alpha antibody; p70-S6K 1 antibody; p70-S6K antibody; P70S6K antibody; P70S6K1 antibody; p70S6Kb antibody; PS6K antibody; Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody; Ribosomal protein S6 kinase beta 1 antibody; Ribosomal protein S6 kinase beta-1 antibody; Ribosomal protein S6 kinase I antibody; RPS6KB1 antibody; S6K antibody; S6K-beta-1 antibody; S6K1 antibody; Serine/threonine kinase 14 alpha antibody; Serine/threonine-protein kinase 14A antibody; STK14A antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

TISSUE SPECIFICITY

Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism (By similarity). Activated by phosphorylation at Thr-252 by PDPK1. Dephosphorylation by PPP1CC at Thr-412 in mitochondrion.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus

FUNCTION

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex.