PRODUCT CODE: EM1701-98

Osteoprotegerin Monoclonal Antibody (EM1701-98)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/1000 dilution.
  • Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/1000 dilution.
  • ICC staining Osteoproteger in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver intestine tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Osteoprotegerin antibody. Counter stained with hematoxylin.
Western blot analysis of Osteoprotegerin on K562 cell lysate using anti-Osteoprotegerin antibody at 1/1000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Osteoprotegerin Monoclonal Antibody (EM1701-98)

Immunogen

Recombinant protein within human osteoprotegerin aa 1-410.

Host

Mouse

Positive Control

K562, MCF-7, rat lung tissue, human liver tissue, human small intestine tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

9C1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

46 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Osteoprotegerin

SYNONYMS

MGC29565 antibody; OCIF antibody; OPG antibody; Osteoclastogenesis inhibitory factor antibody; Osteoprotegerin antibody; PDB5 antibody; TNF receptor superfamily member 11b antibody; TNFRSF 11B antibody; TNFRSF11B antibody; TR 1 antibody; TR1 antibody; TR11B_HUMAN antibody; Tumor necrosis factor receptor superfamily member 11B antibody

TISSUE SPECIFICITY

Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. Contains sialic acid residues.; The N-terminus is blocked.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Bone morphogenesis and remodeling involve the formation of bone from osteoblasts and the resorption of bone by osteoclasts. The cytokine osteoprotegerin (OPG), also designated osteoclastogenesis inhibitory factor (OCIF), is known to inhibit osteoclast formation. A secreted glycoprotein, OPG is a member of the TNF receptor family that increases bone density and volume. OPG is thought to inhibit osteoclastogenesis by disrupting the cell-to-cell signaling between osteoblastic stromal cells and osteoclast progenitors. OPG is known to bind to TRAIL, a death domain-containing protein, and to inhibit TRAIL apoptosis in Jurkat cells. OPG also binds to osteoclast differentiation factor (ODF), also known as TRANCE/RANKL, a membrane-bound protein belonging to the TNF ligand family. Both TNFα and TNFβ upregulate OPG expression, while the bone resorbing agent prostaglandin E2 downregulates OPG.