PRODUCT CODE: ER1802-16

Osteopontin Antibody (ER1802-16)

Applications

  • ICC

  • IHC-P

  • ELISA

REACTIVITY

  • Human

  • Mouse

ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Osteopontin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Osteopontin antibody. Counter stained with hematoxylin.
ICC staining Osteopontin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • ICC

  • IHC-P

  • ELISA

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Osteopontin Antibody (ER1802-16)

Immunogen

Peptide

Host

Rabbit

Positive Control

HepG2, MCF-7, human liver cancer tissue, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • ELISA

  • 1:5,000-1:10,000

TARGET

UNIPROT #

PROTEIN NAME

Osteopontin

SYNONYMS

BNSP antibody; Bone sialoprotein 1 antibody; BSP I antibody; BSPI antibody; Early T lymphocyte activation 1 antibody; ETA 1 antibody; ETA1 antibody; MGC110940 antibody; Nephropontin antibody; OPN antibody; Osteopontin antibody; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody; OSTP_HUMAN antibody; PSEC0156 antibody; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody; Secreted phosphoprotein 1 antibody; SPP 1 antibody; SPP-1 antibody; SPP1 antibody; SPP1/CALPHA1 fusion antibody; Urinary stone protein antibody; Uropontin antibody

SEQUENCE SIMILARITIES

Belongs to the osteopontin family.

TISSUE SPECIFICITY

Bone. Found in plasma.

POST-TRANSLATIONAL MODIFICATION

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Osteopontin (OPN), also designated bone sialoprotein 1, urinary stone protein, spp-1, Eta-1, nephropontin and uropontin, is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPN isolated from or secreted by various tissues ranges in molecular weight due to posttranslational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.