PRODUCT CODE: 0806-8

Osteopontin Antibody (0806-8)

  • Zebrafish

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis on human thymus using anti-Osteopontin polyclonal antibody.
  • Western blot analysis on human thymus using anti-Osteopontin polyclonal antibody.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-8, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0806-8, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis on human thymus using anti-Osteopontin polyclonal antibody.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Osteopontin Antibody (0806-8)

Immunogen

Synthetic peptide within human aa 280-310.

Host

Rabbit

Positive Control

Human breast tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Osteopontin

GENE NAME

SPP1

SEQUENCE SIMILARITIES

Belongs to the osteopontin family.

TISSUE SPECIFICITY

Bone. Found in plasma.

POST-TRANSLATIONAL MODIFICATION

Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif.; O-glycosylated. Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.