PRODUCT CODE: em1510-41

ORM1 Monoclonal Antibody (EM1510-41)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Alpha-1-acid glycoprotein on recombinant protein using anti-Alpha-1-acid glycoprotein antibody at 1/5,000 (1), 1:20,000 (2) dilution.
  • Western blot analysis of Alpha-1-acid glycoprotein on recombinant protein using anti-Alpha-1-acid glycoprotein antibody at 1/5,000 (1), 1:20,000 (2) dilution.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti- Alpha-1-acid glycoprotein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Alpha-1-acid glycoprotein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti- Alpha-1-acid glycoprotein antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti- Alpha-1-acid glycoprotein antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Alpha-1-acid glycoprotein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of Alpha-1-acid glycoprotein on recombinant protein using anti-Alpha-1-acid glycoprotein antibody at 1/5,000 (1), 1:20,000 (2) dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ORM1 Monoclonal Antibody (EM1510-41)

Immunogen

Full length of recombinant protein of human alpha-1-acid glycoprotein.

Host

Mouse

Positive Control

Recombinant protein, rat liver tissue, human lung cancer tissue, mouse liver tissue, human esophagus tissue, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-B10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

24 kDa (Predicted band size)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Alpha-1-acid glycoprotein 1

GENE NAME

ORM1

SYNONYMS

AGP 1, OMD 1, ORM1, AGP1

SEQUENCE SIMILARITIES

Belongs to the calycin superfamily. Lipocalin family.

TISSUE SPECIFICITY

Expressed by the liver and secreted in plasma.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. N-glycan heterogeneity at Asn-33: Hex5HexNAc4 (minor), Hex6HexNAc5 (major) and dHex1Hex6HexNAc5 (minor).

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability in the body. Appears to function in modulating the activity of the immune system during the acute-phase reaction.