PRODUCT CODE: ER1803-12

Orai3 Antibody (ER1803-12)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution.<br />
 Lane 1 : Raji cell lysates<br />
 Lane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)<br />
  • Western blot analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution.<br />
 Lane 1 : Raji cell lysates<br />
 Lane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)<br />
  • ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with Orai3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution.
Lane 1 : Raji cell lysates
Lane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Orai3 Antibody (ER1803-12)

Immunogen

Synthetic peptide within human orai-3 aa 10-50.

Host

Rabbit

Positive Control

Raji, A549, MG-63, SH-SY-5Y, rat brain tissue, human liver cancer tissue, human kidney tissue, human placenta tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Protein orai-3

GENE NAME

ORAI3

SYNONYMS

ORAI3, TMEM142C

SEQUENCE SIMILARITIES

Belongs to the Orai family.

TISSUE SPECIFICITY

Expressed in both naive and effector T helper cells with higher levels in effector cells.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein.

FUNCTION

Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective current by synergy with the Ca(2+) sensor, STIM1.