PRODUCT CODE: ER1803-11

Orai1 Antibody (ER1803-11)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution.
  • Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution.
  • ICC staining Orai1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Orai1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- Orai1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Orai1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MCF-7 cells with Orai1 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Orai1 on PC-3M cell lysate using anti-Orai1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Orai1 Antibody (ER1803-11)

Immunogen

Synthetic peptide within mouse orai1 aa 150-250.

Host

Rabbit

Positive Control

PC-3M, LOVO, MCF-7, human skin tissue, human esophagus tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

33 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Calcium release-activated calcium channel protein 1

GENE NAME

ORAI1

SYNONYMS

ORAI1, CRACM1, TMEM142A

SEQUENCE SIMILARITIES

Belongs to the Orai family.

TISSUE SPECIFICITY

Expressed in naive CD4 and CD8 T cells (at protein level). Expressed at similar levels in naive and effector T helper cells.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated. N-glycosylation inhibits channel activity in T cells.; Ubiquitinated.; Cys-195 is oxidated, leading to inactivation of channel activity.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein. Note=Isoform beta is more mobile in the plasma membrane. Colocalizes with STIM1 at the cell membrane.

FUNCTION

Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.