PRODUCT CODE: ER50105

OOEP Antibody (ER50105)

Applications

  • IHC-P

REACTIVITY

  • Mouse

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-OOEP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-OOEP antibody. Counter stained with hematoxylin.
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-OOEP antibody. Counter stained with hematoxylin.

Applications

  • IHC-P

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

OOEP Antibody (ER50105)

Immunogen

Peptide

Host

Rabbit

Positive Control

Mouse ovary tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

Isotype

IgG

APPLICATION DILUTION

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

Oocyte-expressed protein homolog

GENE NAME

Ooep

SYNONYMS

Floped,mOEP19

SEQUENCE SIMILARITIES

Belongs to the KHDC1 family.

TISSUE SPECIFICITY

Expressed in ovaries, where it is restricted to growing oocytes, with greatest levels in fully grown oocytes.

DEVELOPMENTAL STAGE

Transcripts first detected at 15.5 dpc and peak 1 week after birth. Transcripts accumulate during oogenesis. During meiotic maturation, the vast majority of the transcripts are degraded and virtually none is detected by 2-cell stage embryogenesis. The protein however persists during preimplantation up to the blastocyst stage. At 2-cell stage, excluded from cell-cell contact regions. Continuous exclusion from these regions during preimplantation development leads to the absence of the protein from the inner cells of the morula and the inner cell mass of the blastocyst.

SUBCELLULAR LOCATION

Cytoplasm. Note=In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages. From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions.

FUNCTION

As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.