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PRODUCT CODE: EM100306

OCT-4 Monoclonal Antibody (EM100306)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution.<br /> Positive control: Line 1: NCCIT Line2 :D3
  • Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution.<br /> Positive control: Line 1: NCCIT Line2 :D3
  • ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of OCT-4 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution.
Positive control: Line 1: NCCIT Line2 :D3

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

OCT-4 Monoclonal Antibody (EM100306)

Immunogen

Peptide

Host

Mouse

Positive Control

NCCIT, D3, F9

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A3-C11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

39 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:200

TARGET

UNIPROT #

Q01860

PROTEIN NAME

Oct-4

SYNONYMS

Octamer binding transcription factor 4 antibody; MGC22487 antibody; Oct 3 antibody; Oct 4 antibody; Oct-3 antibody; Oct-4 antibody; OCT3 antibody; Oct4 antibody; Octamer binding protein 3 antibody; Octamer binding protein 4 antibody; Octamer binding transcription factor 3 antibody; Octamer-binding protein 3 antibody; Octamer-binding protein 4 antibody; Octamer-binding transcription factor 3 antibody; OTF 3 antibody; OTF 4 antibody; OTF-3 antibody; OTF3 antibody; OTF4 antibody; PO5F1_HUMAN antibody; POU class 5 homeobox 1 antibody; POU domain class 5 transcription factor 1 antibody; POU domain transcription factor OCT4 antibody; POU domain, class 5, transcription factor 1 antibody; POU-type homeodomain-containing DNA-binding protein antibody; POU5F2 antibody

SEQUENCE SIMILARITIES

Belongs to the POU transcription factor family. Class-5 subfamily.

TISSUE SPECIFICITY

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

DEVELOPMENTAL STAGE

Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

POST-TRANSLATIONAL MODIFICATION

Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.; Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.; ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

Oct-4 (POU5F1) is transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). It forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Oct-4 is critical for early embryogenesis and for embryonic stem cell pluripotency. It is expressed in developing brain and highest levels also found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum.