PRODUCT CODE: ET7108-07

Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64] (ET7108-07)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution.<br />
 Positive control:<br />
 Line 1: K562  <br />
       Line 2: PC-3M<br />
 Line 3: THP-1
  • Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution.<br />
 Positive control:<br />
 Line 1: K562  <br />
       Line 2: PC-3M<br />
 Line 3: THP-1
  • Flow cytometric analysis of A431 cells with Nuf2 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Nuf2 on different cell lysates using anti-Nuf2 at 1/1,000 dilution.
Positive control:
Line 1: K562
Line 2: PC-3M
Line 3: THP-1

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Nuf2 Recombinant Rabbit Monoclonal Antibody [JG34-64] (ET7108-07)

Immunogen

Recombinant protein within human nuf2 aa 1-200.

Host

Rabbit

Positive Control

K562, PC-3M, THP-1, A431.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG34-64

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Nuf2

SYNONYMS

Cancer/testis antigen 106 antibody; CDCA 1 antibody; CDCA1 antibody; Cell division cycle associated 1 antibody; Cell division cycle associated protein 1 antibody; Cell division cycle-associated protein 1 antibody; CT106 antibody; hNuf 2 antibody; hNuf2 antibody; hNuf2R antibody; hsNuf2 antibody; Kinetochore protein Nuf2 antibody; Nuf 2 antibody; NUF2 antibody; NUF2 NDC80 kinetochore complex component antibody; NUF2 NDC80 kinetochore complex component homolog antibody; NUF2, S. cerevisiae, homolog of antibody; NUF2_HUMAN antibody; NUF2R antibody

SEQUENCE SIMILARITIES

Belongs to the NUF2 family.

POST-TRANSLATIONAL MODIFICATION

Can be phosphorylated by AURKA and AURKB.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.