PRODUCT CODE: ER1803-64

NrCAM Antibody (ER1803-64)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NRCAM on SH-SY5Y cell lysate using anti-NRCAM antibody at 1/1,000 dilution.
  • Western blot analysis of NRCAM on SH-SY5Y cell lysate using anti-NRCAM antibody at 1/1,000 dilution.
  • ICC staining NRCAM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NRCAM in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NRCAM in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.
Western blot analysis of NRCAM on SH-SY5Y cell lysate using anti-NRCAM antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NrCAM Antibody (ER1803-64)

Immunogen

Recombinant protein within human nrcam aa 700-900.

Host

Rabbit

Positive Control

SH-SY5Y, A549, SKOV-3, rat brain tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

144 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50 -1:200

TARGET

UNIPROT #

PROTEIN NAME

Neuronal cell adhesion molecule

GENE NAME

NRCAM

SYNONYMS

Nr-CAM, hBravo, Ng-CAM-related, NRCAM, KIAA0343

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

TISSUE SPECIFICITY

Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.

SUBCELLULAR LOCATION

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Secreted. Note=Detected at nodes of Ranvier.

FUNCTION

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.