PRODUCT CODE: ER1802-85

NQO1 Antibody (ER1802-85)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Siha<br />
 Lane 2: A549<br />
  • Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Siha<br />
 Lane 2: A549<br />
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NQO1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Siha cells with NQO1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NQO1 on different cell lysates using anti-NQO1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Siha
Lane 2: A549

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NQO1 Antibody (ER1802-85)

Immunogen

Recombinant protein within human nqo1 1-250 aa.

Host

Rabbit

Positive Control

Siha, A549, rat kidney tissue, mouse colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NAD(P)H dehydrogenase [quinone] 1

GENE NAME

NQO1

SYNONYMS

DTD, QR1, NQO1, DIA4, NMOR1

SEQUENCE SIMILARITIES

Belongs to the NAD(P)H dehydrogenase (quinone) family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

CITATIONS

  • Wang, Wanqiu et al.

    Sinomenine attenuates septic-associated lung injury through the Nrf2-Keap1 and autophagy. | The Journal of Pharmacy and Pharmacology [2020]