NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26] (ET1703-75)
Catalog# ET1703-75
Rabbit Monoclonal to NMDAR1
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WB
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IF-Tissue
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IHC-P
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IHC-Fr
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Human
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Mouse
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Rat
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Cynomolgus monkey
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Pig
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1:500
Antigen retrieval: Not required
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
NMDAR1 Recombinant Rabbit Monoclonal Antibody [JM11-26] (ET1703-75)
Immunogen
Synthetic peptide within human nmdar1 aa 870-910.
Host
Rabbit
Positive Control
MCF7 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, N2A, SHG-44, SH-SY5Y, mouse cerebral cortex tissue, rat cerebral cortex tissue, mouse hippocampus tissue, mouse cerebral cortex tissue, rat cerebral cortex tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-26
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Tissue
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1:200-1:500
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IHC-P
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1:1,000
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IHC-Fr
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 105 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
NMDAR1
SYNONYMS
GluN1 antibody;Glutamate [NMDA] receptor subunit zeta-1 antibody;Glutamate receptor ionotropic N methyl D aspartate 1 antibody;Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody;glutamate receptor ionotropic, NMDA 1 antibody;Grin1 antibody;MRD8 antibody;N methyl D aspartate receptor antibody;N methyl D aspartate receptor channel subunit zeta 1 antibody;N methyl D aspartate receptor subunit NR1 antibody;N-methyl-D-aspartate receptor subunit NR1 antibody;NMD-R1 antibody;NMDA 1 antibody;NMDA R1 antibody;NMDA receptor 1 antibody;NMDA1 antibody;NMDAR antibody;NMDZ1_HUMAN antibody;NR1 antibody
SEQUENCE SIMILARITIES
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
POST-TRANSLATIONAL MODIFICATION
NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
SUBCELLULAR LOCATION
Cell membrane, postsynaptic cell membrane, postsynaptic density.
FUNCTION
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg2+.Sensitivity to glutamate and channel kinetics depend on the subunit composition.
CITATIONS
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Sui Aoran,et al
Scorpion venom heat-resistant synthesized peptide ameliorates epileptic seizures and imparts neuroprotection in rats mediated by NMDA receptors
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WB
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Rat
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Citation
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