Positive control:
Lane 1: K562 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: 293 cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
NM23 Monoclonal Antibody (EM1901-09)
Immunogen
Synthetic peptide within human nm23 aa 1-90.
Host
Mouse
Positive Control
K562 cell, A549 cell, HepG2 cell, 293 cell, human liver carcinoma tissue, human thyroid tissue, human skin tissue, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
13C1
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
17/19 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:500-1:2,000
-
IHC-P:1:50-1:100
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
NM23
SYNONYMS
AWD antibody; AWD, drosophila, homolog of antibody; GAAD antibody; Granzyme A activated DNase antibody; Granzyme A-activated DNase antibody; GZMA activated DNase antibody; Metastasis inhibition factor NM23 antibody; NB antibody; NBS antibody; NDK A antibody; NDKA antibody; NDKA_HUMAN antibody; NDP kinase A antibody; NDPK-A antibody; NDPKA antibody; NM23 antibody; NM23 long variant, included antibody; nm23-H1 antibody; NM23-M1 antibody; NM23H1B, included antibody; NME/NM23 nucleoside diphosphate kinase 1 antibody; Nme1 antibody; NME1-NME2 spliced read-through transcript, included antibody; Non-metastatic cells 1, protein (NM23A) expressed in antibody; Nonmetastatic cells 1, protein expressed in antibody; Nonmetastatic protein 23 antibody; Nonmetastatic protein 23, homolog 1 antibody; Nucleoside diphosphate kinase A antibody; Tumor metastatic process-associated protein antibody
SEQUENCE SIMILARITIES
Belongs to the NDK family.
TISSUE SPECIFICITY
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.