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PRODUCT CODE: ER1803-46

Nfic Antibody (ER1803-46)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of Nfic on different cell lysate using anti-Nfic antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: A549<br /> Lane 2: SiHa
  • Western blot analysis of Nfic on different cell lysate using anti-Nfic antibody at 1/500 dilution.<br /> Positive control:<br /> Lane 1: A549<br /> Lane 2: SiHa
  • ICC staining Nfic in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nfic in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Nfic in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Nfic antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
  • Flow cytometric analysis of SiHa cells with Nfic antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Nfic on different cell lysate using anti-Nfic antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: SiHa

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Nfic Antibody (ER1803-46)

Immunogen

Synthetic peptide within human nfic 400-450 aa.

Host

Rabbit

Positive Control

A549, SiHa, A431.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P08651

PROTEIN NAME

Nfic

SYNONYMS

1110019L22Rik antibody; 1500041O16Rik antibody; AA589446 antibody; AI746521 antibody; CAAT box transcription factor antibody; CCAAT binding transcription factor antibody; CCAAT box binding transcription factor antibody; CCAAT-box-binding transcription factor antibody; CNFI C antibody; CTF antibody; CTF5 antibody; MGC137374 antibody; MGC20153 antibody; NF I antibody; NF I/C antibody; NF-I/C antibody; NF1 C antibody; NF1-C antibody; NF1C antibody; NFI antibody; NFI-C antibody; NFI/C antibody; NFIC antibody; NFIC_HUMAN antibody; Nuclear factor 1 antibody; Nuclear factor 1 C type antibody; Nuclear factor 1 C-type antibody; Nuclear factor 1/C antibody; Nuclear factor I/C (CCAAT binding transcription factor) antibody; Nuclear factor I/C antibody; TGGCA binding protein antibody; TGGCA-binding protein antibody; Transcription factor NFIC antibody

SEQUENCE SIMILARITIES

Belongs to the CTF/NF-I family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.