PRODUCT CODE: EM1701-89

NFIB/NF1B2 Monoclonal Antibody (EM1701-89)

Applications

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.

Applications

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

NFIB/NF1B2 Monoclonal Antibody (EM1701-89)

Immunogen

Recombinant full length protein corresponding to human nfib/nf1b2.

Host

Mouse

Positive Control

Rat heart tissue, human colon cancer tissue, human prostate tissue, human pancreas tissue, mouse liver tissue, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1H1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Nuclear factor 1 B-type

GENE NAME

NFIB

SYNONYMS

NF1-B, Nuclear factor 1/B, CTF, NF-I/B, NFI-B, NFIB

SEQUENCE SIMILARITIES

Belongs to the CTF/NF-I family.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.