PRODUCT CODE: 0805-3

Neuron-specific class III beta-tubulin Antibody (0805-3)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Tubulin beta-3 chain on Hela cell lysate using anti-Tubulin beta-3 chain antibody at 1/10,000 dilution.
  • Western blot analysis of Tubulin beta-3 chain on Hela cell lysate using anti-Tubulin beta-3 chain antibody at 1/10,000 dilution.
  • ICC staining Tubulin beta-3 chain in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Tubulin beta-3 chain in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Tubulin beta-3 chain antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Tubulin beta-3 chain antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Tubulin beta-3 chain on Hela cell lysate using anti-Tubulin beta-3 chain antibody at 1/10,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Neuron-specific class III beta-tubulin Antibody (0805-3)

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (klh-coupled) corresponding to a region of n-terminal residues of tubulin beta-3.

Host

Rabbit

Positive Control

Hela, HepG2, NIH-3T3, rat brain tissue, rat kidney tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:5,000-1:10,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:100-1:400

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Neuron-specific class Ⅲ β-tubulin

SYNONYMS

beta 3 tubulin antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M(beta)3 antibody; M(beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific class III beta-tubulin antibody; QccE-11995 antibody; QccE-15186 antibody; TBB3_HUMAN antibody; Tubb 3 antibody; TUBB3 antibody; TUBB4 antibody; Tubulin beta 3 antibody; Tubulin beta 3 chain antibody; Tubulin beta 4 antibody; Tubulin beta III antibody; Tubulin beta-3 chain antibody; Tubulin beta-4 chain antibody; Tubulin beta-III antibody

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

TISSUE SPECIFICITY

Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

SUBCELLULAR LOCATION

Cytoplasm. Cytoskeleton. Microtubule.

FUNCTION

Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.