PRODUCT CODE: ET7110-59

Neurocan Recombinant Rabbit Monoclonal Antibody [JE54-15] (ET7110-59)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

-
+
Western blot analysis of Neurocan on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Neurocan on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Neurocan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Neurocan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7110-59, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Neurocan on SiHa cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Neurocan Recombinant Rabbit Monoclonal Antibody [JE54-15] (ET7110-59)

Immunogen

Synthetic peptide within n-terminal human neurocan.

Host

Rabbit

Positive Control

SiHa cell lysates, human liver carcinoma tissue, human brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE54-15

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

Predicted band size 143 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Neurocan

SYNONYMS

Chondroitin sulfate proteoglycan 3 antibody; Cspg3 antibody; Ncan antibody; NCAN_HUMAN antibody; NEUR antibody; Neurocan core protein antibody; Neurocan proteoglycan antibody

SEQUENCE SIMILARITIES

Belongs to the aggrecan/versican proteoglycan family.

TISSUE SPECIFICITY

Brain.

POST-TRANSLATIONAL MODIFICATION

O-glycosylated.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Neurocan core protein is a protein that in humans is encoded by the NCAN gene. Neurocan is a member of the lectican / chondroitin sulfate proteoglycan protein families and consists of neurocan core protein and chondroitin sulfate. It is thought to be involved in the modulation of cell adhesion and migration. Neurocan is a significant component of the extracellular matrix, and its levels are modulated by a variety of factors, but mice in which the NCAN gene has been knocked out show no easily observable defects in brain development or behavior. However, a genome-wide association study published in 2011 identified Neurocan as a susceptibility factor for bipolar disorder. A more comprehensive study published in 2012 confirmed that association. The 2012 study examined correlations between NCAN alleles and various symptoms of bipolar disorder, and also examined the behavior of NCAN knockout mice. In the human subjects, it was found that NCAN genotype was strongly associated with manic symptoms but not with depressive symptoms. In the mice, the absence of functional Neurocan resulted in a variety of manic-like behaviors, which could be normalized by administering lithium.