PRODUCT CODE: ER1803-67

Nephrin Antibody (ER1803-67)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Rat

Western blot analysis of Nephrin on human kidney lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Nephrin on human kidney lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining Nephrin in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Nephrin polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining Nephrin in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Nephrin polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining Nephrin in PC-12 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Nephrin polyclonal antibody at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Nephrin on human kidney lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Nephrin Antibody (ER1803-67)

Immunogen

Recombinant protein within human nephrin aa 1000-1250.

Host

Rabbit

Positive Control

Human kidney tissue, 293T, PANC-1, PC-12.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

160 kDa, predicted band size 136 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • ICC:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Nephrin

SYNONYMS

CNF antibody; Nephrin antibody; Nephrosis 1 congenital Finnish type antibody; Nephrosis 1, congenital, Finnish type (nephrin) antibody; NPHN antibody; NPHN_HUMAN antibody; NPHS 1 antibody; Nphs1 antibody; Renal glomerulus specific cell adhesion receptor antibody; Renal glomerulus-specific cell adhesion receptor antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily.

TISSUE SPECIFICITY

Specifically expressed in podocytes of kidney glomeruli.

DEVELOPMENTAL STAGE

In 23-week-old embryo found in epithelial podocytes of the periphery of mature and developing glomeruli.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1.

SUBCELLULAR LOCATION

Cell membrane, Membrane.

FUNCTION

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The renal filtration barrier consists of fenestrated endothelial cells, the glomerular basement membrane, and the podocytes of epithelial cells. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes. A defect in the gene for nephrin, NPHS1, is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, or proteinuria. Nephrin is also required for cardiovascular development. Plays a role in skeletal muscle formation through regulation of myoblast fusion.