Applications
-
WB
-
ICC
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
NEDD8 Antibody (ER1802-5)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
PC-3M, HepG2, MCF-7.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein affinity purified
MOLECULAR WEIGHT
9 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC
-
1:50-1:100
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
NEDD8
SYNONYMS
FLJ43224 antibody; MGC104393 antibody; MGC125896 antibody; MGC125897 antibody; NED8 antibody; NEDD 8 antibody; NEDD-8 antibody; Nedd8 antibody; NEDD8_HUMAN antibody; Neddylin antibody; Neural precursor cell expressed developmentally down regulated 8 antibody; Neural precursor cell expressed developmentally down regulated gene 8 antibody; Neural precursor cell expressed developmentally down-regulated protein 8 antibody; Rub1 antibody; Ubiquitin like protein Nedd 8 antibody; Ubiquitin like protein Nedd8 antibody; Ubiquitin-like protein Nedd8 antibody
SEQUENCE SIMILARITIES
Belongs to the ubiquitin family.
TISSUE SPECIFICITY
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
POST-TRANSLATIONAL MODIFICATION
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
NEDD8 is a ubiquitin (Ub)-like molecule that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog Rub1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, Ubc12. NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein, von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S Proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways.