PRODUCT CODE: ER1802-5

NEDD8 Antibody (ER1802-5)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of NEDD8 on PC-3M cell lysate using anti-NEDD8 antibody at 1/1,000 dilution.<br />
  • Western blot analysis of NEDD8 on PC-3M cell lysate using anti-NEDD8 antibody at 1/1,000 dilution.<br />
  • ICC staining NEDD8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NEDD8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NEDD8 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of MCF-7 cells with NEDD8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of NEDD8 on PC-3M cell lysate using anti-NEDD8 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NEDD8 Antibody (ER1802-5)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-3M, HepG2, MCF-7.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

9 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

NEDD8

GENE NAME

NEDD8

SYNONYMS

NEDD-8, NEDD8

SEQUENCE SIMILARITIES

Belongs to the ubiquitin family.

TISSUE SPECIFICITY

Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.

POST-TRANSLATIONAL MODIFICATION

Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.

SUBCELLULAR LOCATION

Nucleus. Note=Mainly nuclear.

FUNCTION

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.