PRODUCT CODE: em1701-35

NaV1.7 Monoclonal Antibody (EM1701-35)

Applications

  • IHC-P

  • ICC

  • FC

  • WB

REACTIVITY

  • Human

ICC staining NaV1.7 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NaV1.7 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining NaV1.7 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with NaV1.7 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
ICC staining NaV1.7 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • IHC-P

  • ICC

  • FC

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

NaV1.7 Monoclonal Antibody (EM1701-35)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

A549, Hela, human colon cancer tissue, human kidney tissue, A549.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1D6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

226 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:200

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

Sodium channel protein type 9 subunit alpha

GENE NAME

SCN9A

SYNONYMS

hNE-Na, PN1, SCN9A, NENA

SEQUENCE SIMILARITIES

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.

TISSUE SPECIFICITY

Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.; Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4.

SUBCELLULAR LOCATION

Cell membrane; Multi-pass membrane protein. Cell projection, neuron projection. Note=In neurite terminals.

FUNCTION

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-sensitive Na(+) channel isoform. Plays a role in pain mechanisms, especially in the development of inflammatory pain.