PRODUCT CODE: R1511-31

NADPH oxidase 4 Antibody (R1511-31)

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Immunocytochemical staining of HepG2 cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.
  • Immunocytochemical staining of HepG2 cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.
  • Immunocytochemical staining of 293T cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.
  • Immunocytochemical staining of Hela cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-NADPH oxidase 4 rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-NADPH oxidase 4 rabbit polyclonal antibody.
  • Flow cytometric analysis of Hela cells with NADPH oxidase 4 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemical staining of HepG2 cells using anti-NADPH oxidase 4 rabbit polyclonal antibody.

Applications

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

NADPH oxidase 4 Antibody (R1511-31)

Immunogen

Peptide

Host

Rabbit

Positive Control

HepG2, 293T, Hela, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:10-1:100

TARGET

UNIPROT #

PROTEIN NAME

NADPH oxidase 4

SYNONYMS

Kidney oxidase 1 antibody; Kidney oxidase-1 antibody; Kidney superoxide producing NADPH oxidase antibody; Kidney superoxide-producing NADPH oxidase antibody; KOX 1 antibody; KOX antibody; Kox-1 antibody; KOX1 antibody; NADPH antibody; NADPH oxidase 4 antibody; Nox4 antibody; NOX4_HUMAN antibody; Renal NAD(P)H oxidase antibody; Renal NAD(P)H-oxidase antibody; RENOX antibody

TISSUE SPECIFICITY

Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.

DEVELOPMENTAL STAGE

Expressed in fetal kidney and fetal liver.

POST-TRANSLATIONAL MODIFICATION

Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

SUBCELLULAR LOCATION

Cell membrane, Nucleus

FUNCTION

The superoxide-generating NADPH oxidase includes a membrane-bound flavocytochrome containing two subunits, gp91-phox and p22-phox, and the cytosolic proteins p47-phox and p67-phox. During activation of the NADPH oxidase, p47-phox and p67-phox migrate to the plasma membrane where they associate with the flavocytochrome, cytochrome b558, to form the active enzyme complex. The p22 and gp91-phox subunits also function as surface O2 sensors that initiate cellular signaling in response to hypoxic conditions. Nox4 (also known as Renox) is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of Angiotensin II increases Nox4 expression, which suggests a role for Nox4 in vascular oxidative stress response. The gene encoding human Nox4 maps to chromosome 11q14.2-q21