PRODUCT CODE: ER0503

N-Cadherin Rabbit Polyclonal Antibody (ER0503)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Mouse heart lysates<br />
Lane2 : Human heart lysates
  • Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Mouse heart lysates<br />
Lane2 : Human heart lysates
  • ICC staining N-Cadherin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining N-Cadherin in mouse embryonic stem cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse heart lysates
Lane2 : Human heart lysates

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

N-Cadherin Rabbit Polyclonal Antibody (ER0503)

Immunogen

Synthetic peptide (klh-coupled) within human n-cadherin 360-420 aa.

Host

Rabbit

Positive Control

Mouse heart, human heart, 293, mouse embryonic stem cell, HepG2 Human colon?carcinoma tissue, human liver?carcinoma tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

140 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

N-Cadherin

SYNONYMS

CADH2_HUMAN antibody; Cadherin 2 antibody; Cadherin 2 N cadherin neuronal antibody; Cadherin 2 type 1 antibody; Cadherin 2 type 1 N cadherin neuronal antibody; Cadherin 2, type 1, N-cadherin (neuronal) antibody; Cadherin-2 antibody; Cadherin2 antibody; Calcium dependent adhesion protein neuronal antibody; CD325 antibody; CD325 antigen antibody; CDH2 antibody; CDHN antibody; CDw325 antibody; CDw325 antigen antibody; N cadherin 1 antibody; N-cadherin antibody; NCAD antibody; Neural cadherin antibody; OTTHUMP00000066304 antibody; OTTHUMP00000067378 antibody

POST-TRANSLATIONAL MODIFICATION

Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).; May be phosphorylated by OBSCN.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

The cadherins are a large family of cell adhesion molecules involved in calcium-dependent recognition and adhesion events. The cadherin subfamily includes N-, P-, B-, and E- cadherins, and other members. Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. N-Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. When a cancer cell adheres to the endothelial cells of a blood vessel it up-regulates the src kinase pathway, which phosphorylates beta-catenins attached to both N-cadherin (this protein) and E-cadherins.