PRODUCT CODE: ER0503

N-Cadherin Antibody (ER0503)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Mouse heart lysates<br />
Lane2 : Human heart lysates
  • Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.<br />
Positive control: <br />
Lane 1: Mouse heart lysates<br />
Lane2 : Human heart lysates
  • ICC staining N-Cadherin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining N-Cadherin in mouse embryonic stem cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining N-Cadherin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-N-Cadherin antibody. Counter stained with hematoxylin.
Western blot analysis of N-cadherin on different tissue lysates using anti- N-cadherin antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse heart lysates
Lane2 : Human heart lysates

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

N-Cadherin Antibody (ER0503)

Immunogen

Synthetic peptide (klh-coupled) within human n-cadherin 360-420 aa.

Host

Rabbit

Positive Control

Mouse heart, human heart, 293, mouse embryonic stem cell, HepG2 Human colon?carcinoma tissue, human liver?carcinoma tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

140 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

Cadherin-2

GENE NAME

CDH2

SYNONYMS

N-cadherin,CADH2

POST-TRANSLATIONAL MODIFICATION

Cleaved by MMP24. Ectodomain cleavage leads to the generation of a soluble 90 kDa amino-terminal soluble fragment and a 45 kDa membrane-bound carboxy-terminal fragment 1 (CTF1), which is further cleaved by gamma-secretase into a 35 kDa. Cleavage in neural stem cells by MMP24 affects CDH2-mediated anchorage of neural stem cells to ependymocytes in the adult subependymal zone, leading to modulate neural stem cell quiescence (By similarity).; May be phosphorylated by OBSCN.

SUBCELLULAR LOCATION

Cell membrane. Cell surface. Note=Colocalizes with TMEM65 at the intercalated disk in cardiomyocytes. Colocalizes with OBSCN at the intercalated disk and at sarcolemma in cardiomyocytes.

FUNCTION

Calcium-dependent cell adhesion protein; preferentially mediates homotypic cell-cell adhesion by dimerization with a CDH2 chain from another cell. Cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.