PRODUCT CODE: EM1701-29

MTERFD1 Monoclonal Antibody (EM1701-29)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of MTERFD1 on different lysates using anti-MTERFD1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: Mouse kidney tissue<br />
 Lane 3: K562
  • Western blot analysis of MTERFD1 on different lysates using anti-MTERFD1 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Hela<br />
 Lane 2: Mouse kidney tissue<br />
 Lane 3: K562
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTERFD1 antibody. Counter stained with hematoxylin.
Western blot analysis of MTERFD1 on different lysates using anti-MTERFD1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse kidney tissue
Lane 3: K562

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

MTERFD1 Monoclonal Antibody (EM1701-29)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

Hela, mouse kidney tissue lysate, K562, human liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A8-A10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

48/38 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

  • ICC

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

MTERFD1

SYNONYMS

CGI 12 antibody; mitochondrial antibody; Mitochondrial transcription termination factor 3 antibody; MTER1_HUMAN antibody; MTERF domain containing 1 antibody; mTERF domain containing protein 1 mitochondrial antibody; mTERF domain-containing protein 1 antibody; mTERF3 antibody; Mterfd1 antibody; Transcription termination factor 3, mitochondrial antibody

SEQUENCE SIMILARITIES

Belongs to the mTERF family.

TISSUE SPECIFICITY

Highly expressed in heart, liver, kidney and testis. Detected at lower levels in brain, spleen and lung.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

MTERFD1 belongs to the MTERF family which consists of four members including MTERF1, MTERF2, MTERF3 and MTERF4. Previous research showed that MTERFD1 is a mitochondrial protein that interacts with the mitochondrial DNA promoter region and decreases transcription initiation in mammalian mitochondria. This negative regulation is likely important for fine-tuning mitochondria transcription in response to physiological demands. Human mitochondrial transcription termination factor domain 1 (MTERFD1) was mutated in many types of cancer. In vivo and in vitro data indicated that MTERFD1 gene possesses oncogenic properties.