Applications
-
WB
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
MSY2 Monoclonal Antibody (EM1701-40)
Immunogen
Peptide.
Host
Mouse
Positive Control
MCF-7, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A2-B9-G6
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G purified.
MOLECULAR WEIGHT
38.5 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
MSY2
SYNONYMS
Contrin antibody; CSDA 3 antibody; CSDA3 antibody; Dbpc antibody; DNA binding protein C antibody; DNA-binding protein C antibody; FRGY2 homolog antibody; Germ cell specific Y box binding protein antibody; Germ cell-specific Y-box-binding protein antibody; MGC118270 antibody; MGC45104 antibody; MSY 2 antibody; MSY2 antibody; MSY2 homolog antibody; OTTMUSP00000006276 antibody; RGD1305068 antibody; Y box binding protein 2 antibody; Y-box-binding protein 2 antibody; YBOX2_HUMAN antibody; YBX 2 antibody; YBX2 antibody
TISSUE SPECIFICITY
Expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Also observed placental trophoblasts, as well as in vascular smooth muscle cells in the pulmonary artery, myocardium, and skeletal muscle. Undetectable in epithelial cells in respiratory, gastrointestinal, and urogenital tracts. Up-regulated in various carcinomas and germ cell tumors (at protein level).
POST-TRANSLATIONAL MODIFICATION
Phosphorylated during oocyte maturation and dephosphorylated following egg activation. Phosphorylated in vitro by a kinase activity associated with testicular mRNPs. Dephosphorylation leads to a decrease in its affinity to bind RNA in vitro (By similarity).
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full-length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay.