PRODUCT CODE: EM1701-40

MSY2 Monoclonal Antibody (EM1701-40)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MYS2 on MCF-7 cell (1) and mouse testis tissue lysate using anti-MSY2 antibody at 1/1,000 dilution.
  • Western blot analysis of MYS2 on MCF-7 cell (1) and mouse testis tissue lysate using anti-MSY2 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MSY2 antibody. Counter stained with hematoxylin.
Western blot analysis of MYS2 on MCF-7 cell (1) and mouse testis tissue lysate using anti-MSY2 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

MSY2 Monoclonal Antibody (EM1701-40)

Immunogen

Peptide.

Host

Mouse

Positive Control

MCF-7, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-B9-G6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

38.5 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

MSY2

SYNONYMS

Contrin antibody; CSDA 3 antibody; CSDA3 antibody; Dbpc antibody; DNA binding protein C antibody; DNA-binding protein C antibody; FRGY2 homolog antibody; Germ cell specific Y box binding protein antibody; Germ cell-specific Y-box-binding protein antibody; MGC118270 antibody; MGC45104 antibody; MSY 2 antibody; MSY2 antibody; MSY2 homolog antibody; OTTMUSP00000006276 antibody; RGD1305068 antibody; Y box binding protein 2 antibody; Y-box-binding protein 2 antibody; YBOX2_HUMAN antibody; YBX 2 antibody; YBX2 antibody

TISSUE SPECIFICITY

Expressed in oocytes and testicular germ cells in the stage of spermatogonia to spermatocyte. Also observed placental trophoblasts, as well as in vascular smooth muscle cells in the pulmonary artery, myocardium, and skeletal muscle. Undetectable in epithelial cells in respiratory, gastrointestinal, and urogenital tracts. Up-regulated in various carcinomas and germ cell tumors (at protein level).

POST-TRANSLATIONAL MODIFICATION

Phosphorylated during oocyte maturation and dephosphorylated following egg activation. Phosphorylated in vitro by a kinase activity associated with testicular mRNPs. Dephosphorylation leads to a decrease in its affinity to bind RNA in vitro (By similarity).

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full-length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay.