PRODUCT CODE: ER2001-38

mSin3A Antibody (ER2001-38)

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis of mSin3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: HL-60 cell lysate<br />
 Lane 2: A549 cell lysate<br />
 Lane 3: Siha cell lysate
  • Western blot analysis of mSin3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
 Positive control: <br />
 Lane 1: HL-60 cell lysate<br />
 Lane 2: A549 cell lysate<br />
 Lane 3: Siha cell lysate
Western blot analysis of mSin3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: A549 cell lysate
Lane 3: Siha cell lysate

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

mSin3A Antibody (ER2001-38)

Immunogen

Synthetic peptide within human msin3a aa 1-50.

Host

Rabbit

Positive Control

HL-60 cell lysate, A549 cell lysate, Siha cell lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

145 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

mSin3A

SYNONYMS

AW553200 antibody; DKFZP434K2235 antibody; FLJ90319 antibody; Histone deacetylase complex subunit Sin 3a antibody; Histone deacetylase complex subunit Sin3a antibody; KIAA0700 antibody; Kiaa4126 antibody; mKIAA4126 antibody; Paired amphipathic helix protein Sin 3a antibody; Paired amphipathic helix protein Sin3a antibody; Sin 3a antibody; SIN3 homolog A antibody; SIN3 homolog A transcription regulator (yeast) antibody; SIN3 homolog A transcription regulator antibody; SIN3 transcription regulator homolog A antibody; Sin3a antibody; SIN3A protein antibody; SIN3A_HUMAN antibody; Transcriptional co repressor Sin 3A antibody; Transcriptional co repressor Sin3A antibody; Transcriptional corepressor Sin3a antibody; Transcriptional regulator SIN3A antibody

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).