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PRODUCT CODE: ER1706-59

MSI2 Antibody (ER1706-59)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of MSI2 on SW480 (1) and K562 (2) cell lysate using anti-MSI2 antibody at 1/500 dilution.
  • Western blot analysis of MSI2 on SW480 (1) and K562 (2) cell lysate using anti-MSI2 antibody at 1/500 dilution.
  • ICC staining MSI2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MSI2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with MSI2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of MSI2 on SW480 (1) and K562 (2) cell lysate using anti-MSI2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MSI2 Antibody (ER1706-59)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

SW480, K562, HepG2, PC-12, SH-SY5Y, rat epididymis tissue, human breast tissue, human placenta tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

35/37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q96DH6

PROTEIN NAME

MSI2

SYNONYMS

FLJ36569 antibody; MGC3245 antibody; Msi2 antibody; MSI2/HOXA9 fusion gene, included antibody; MSI2H antibody; MSI2H_HUMAN antibody; Musashi 2 antibody; Musashi homolog 2 antibody; Musashi RNA binding protein 2 antibody; Musashi, Drosophila, homolog of, 2 antibody; Musashi-2 antibody; RNA binding protein Musashi homolog 2 antibody; RNA-binding protein Musashi homolog 2 antibody; WD 40 repeat protein MSI2 antibody

SEQUENCE SIMILARITIES

Belongs to the Musashi family.

TISSUE SPECIFICITY

Ubiquitous; detected at low levels.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Msi2 (musashi homolog 2), also known as MSI2H, is a 328 amino acid protein that localizes to the cytoplasm and contains two RRM (RNA recognition motif) domains. Expressed ubiquitously at low levels, Msi2 functions as an RNA binding protein that, by regulating the expression of target mRNAs, is thought to play a role in the proliferation and maintenance of stem cells within the central nervous system. Msi2 is subject to post-translational phosphorylation and is upregulated in response to brain injury, suggesting a role in healing and brain tissue regeneration. Chromosomal aberrations involving the Msi2 gene are associated with the progression of chronic myeloid leukemia. Multiple isoforms of Msi2 exist due to alternative splicing events.