Lane 1: human skin tissue lysate
Lane 2: rat brain tissue lysate
Mouse monoclonal primary
MSH6 Monoclonal Antibody (EM1902-24)
Synthetic peptide corresponding to c terminal human msh6.
Rat brain lysates, human thyroid tissue, human colon carcinoma tissue, human skin tissue, human breast carcinoma tissue, human esophagus tissue, human placenta tissue, mouse testis tissue, mouse colon tissue, mouse placenta tissue, K562.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein G affinity purified.
DNA mismatch repair protein Msh6
Belongs to the DNA mismatch repair MutS family.
The N-terminus is blocked.; Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.
Nucleus. Chromosome. Note=Associates with H3K36me3 via its PWWP domain.
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.