PRODUCT CODE: ER1706-40

MMP9 Antibody (ER1706-40)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MMP9 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MMP9 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MMP9 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-MMP9 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP9 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HL-60 cells with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Goat anti rabbit IgG (FITC) was used as the secondary antibody
ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MMP9 Antibody (ER1706-40)

Immunogen

Peptide

Host

Rabbit

Positive Control

U937 cell lysates, Hela, MCF-7, PANC-1, human spleen tissue, mouse spleen tissue, human tonsil tissue, HL-60.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • WB

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

MMP9

SYNONYMS

82 kDa matrix metalloproteinase-9 antibody; 92 kDa gelatinase antibody; 92 kDa type IV collagenase antibody; CLG 4B antibody; CLG4B antibody; Collagenase Type 4 beta antibody; Collagenase type IV 92 KD antibody; EC 3.4.24.35 antibody; Gelatinase 92 KD antibody; Gelatinase B antibody; Gelatinase beta antibody; GelatinaseB antibody; GELB antibody; Macrophage gelatinase antibody; MANDP2 antibody; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) antibody; Matrix Metalloproteinase 9 antibody; MMP 9 antibody; MMP-9 antibody; MMP9 antibody; MMP9_HUMAN antibody; Type V collagenase antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase M10A family.

TISSUE SPECIFICITY

Detected in neutrophils (at protein level). Produced by normal alveolar macrophages and granulocytes.

POST-TRANSLATIONAL MODIFICATION

Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.; N- and O-glycosylated.

SUBCELLULAR LOCATION

Extracellular matrix. Secreted.

FUNCTION

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.