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PRODUCT CODE: ER31211

MMP1 Antibody (ER31211)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution.<br /> Positive control: <br /> Lane 1: SKBR3 <br /> Lane 2: Raji <br /> Lane 3: A431 <br /> Lane 4: A549
  • Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution.<br /> Positive control: <br /> Lane 1: SKBR3 <br /> Lane 2: Raji <br /> Lane 3: A431 <br /> Lane 4: A549
  • ICC staining MMP1 in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP1 antibody. Counter stained with hematoxylin.
Western blot analysis of MMP1 on different cell lysates using anti-MMP1 antibody at 1/1000 dilution.
Positive control:
Lane 1: SKBR3
Lane 2: Raji
Lane 3: A431
Lane 4: A549

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MMP1 Antibody (ER31211)

Immunogen

Peptide

Host

Rabbit

Positive Control

A549, MCF-7, Raji, SKBR3, A431, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

P03956

PROTEIN NAME

Interstitial collagenase

GENE NAME

MMP1

SYNONYMS

MMP-1

SEQUENCE SIMILARITIES

Belongs to the peptidase M10A family.

POST-TRANSLATIONAL MODIFICATION

Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.; Tyrosine phosphorylated in platelets by PKDCC/VLK.

SUBCELLULAR LOCATION

Secreted, extracellular space, extracellular matrix.

FUNCTION

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

CITATIONS

  • Wang, Ji et al.

    Matrix metalloproteinase-1 expression in breast carcinoma: a marker for unfavorable prognosis. | Oncotarget [2017]