PRODUCT CODE: ER1706-77

MMP-3 Antibody (ER1706-77)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of MMP-3 on LOVO cell lysate using anti-MMP-3 antibody at 1/200 dilution.
  • Western blot analysis of MMP-3 on LOVO cell lysate using anti-MMP-3 antibody at 1/200 dilution.
  • ICC staining MMP-3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MMP-3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with MMP-3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of MMP-3 on LOVO cell lysate using anti-MMP-3 antibody at 1/200 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MMP-3 Antibody (ER1706-77)

Immunogen

Peptide.

Host

Rabbit

Positive Control

A549, LOVO, rat brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

54 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Stromelysin-1

GENE NAME

MMP3

SYNONYMS

SL-1, MMP-3, MMP3, STMY1

SEQUENCE SIMILARITIES

Belongs to the peptidase M10A family.

SUBCELLULAR LOCATION

Secreted, extracellular space, extracellular matrix.

FUNCTION

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.