PRODUCT CODE: ER40104

MGMT Antibody (ER40104)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7 <br />
 Lane 3: Daudi <br />
 Lane 4: Raji <br />
 Lane 5: SK-BR-3 <br />
 Lane 6: SKOV3 <br />
 Lane 7: Human liver <br />
 Lane 8: Human kidney <br />
 Lane 9: Human thymus <br />
 Lane 10: Human placentae
  • Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution.<br />
 Positive control: <br />
 Lane 1: Hela <br />
 Lane 2: MCF-7 <br />
 Lane 3: Daudi <br />
 Lane 4: Raji <br />
 Lane 5: SK-BR-3 <br />
 Lane 6: SKOV3 <br />
 Lane 7: Human liver <br />
 Lane 8: Human kidney <br />
 Lane 9: Human thymus <br />
 Lane 10: Human placentae
  • ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MGMT in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining MGMT in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MGMT antibody. Counter stained with hematoxylin.
Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: MCF-7
Lane 3: Daudi
Lane 4: Raji
Lane 5: SK-BR-3
Lane 6: SKOV3
Lane 7: Human liver
Lane 8: Human kidney
Lane 9: Human thymus
Lane 10: Human placentae

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

MGMT Antibody (ER40104)

Immunogen

Peptide

Host

Rabbit

Positive Control

MCF-7, Hela, Dauai, Raji, A172,human kidney tissue, human liver tissue, human thymus tissue, human placentae tissue, Jurkat,SK-BR-3 ,SKOV3,human tonsil tissuehuman colon carcinoma tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

22 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC:1:100

TARGET

UNIPROT #

PROTEIN NAME

MGMT

SYNONYMS

6 O methylguanine DNA methyltransferase antibody; 6-O-methylguanine-DNA methyltransferase antibody; Agat antibody; AGT antibody; AI267024 antibody; EC 2.1.1.63 antibody; Methylated DNA protein cysteine methyltransferase antibody; Methylated-DNA--protein-cysteine methyltransferase antibody; Methylguanine DNA methyltransferase antibody; MGC107020 antibody; MGMT antibody; MGMT_HUMAN antibody; O 6 methylguanine DNA alkyltransferase antibody; O 6 methylguanine DNA methyltransferase antibody; O-6-methylguanine-DNA methyltransferase antibody; O-6-methylguanine-DNA-alkyltransferase antibody

SEQUENCE SIMILARITIES

Belongs to the MGMT family.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.